3/17/2024 0 Comments Rotor gene corbett 6000from cycling to melt) “Add 5 cycles” lets you add cycles at the endĪdding sample names and types Can highlight blocks of cells for naming etc (like excel) Samples with the same name will be treated as replicates regardless of position Type can be standard, no template control, unknown (sample) Click on “Edit Samples” on RHS of screenĭrop down “Type” menu to view options If you choose “Standard” you have to add a value for concentration If you are running two standards define one set on page 1 then make a new page (page 2) to define the second set of standards Groups can be used to define e.g HKG (housekeeper) and GOI (gene of interestĬan choose colour from palette and can set colour gradient across range of samples/standards “Skip” allows you to skip to next step (e.g. Choose to enter sample names now or click “Finish” and enter sample names later Run will commence, while the experiment is running you will see: Raw Channel - fluorescence collected cycle by cycle Temperature - profile as temperature cycles Profile Progress – tells you time remaining, shows you where you are. ![]() To save as a template open template folder C:\Program Files\Rotor-Gene 6000 Software\Templates and save as template file (*.ret) Run files are *.rex Gain can be compared to the aperture on a camera and is used to fit data on raw scale If your starting fluorescence signal is weak the gain should be high, if strong the gain should be low Many people just accept default gain and go to “Next” on this screen, others optimise the gain for each run Click on “Gain Optimisation” to beginĬlick on “Optimise Acquiring” and “Perform Calibration Before 1st Acquisition” If you have different colours in different positions click “Edit” The colour you want to use must be in the tube position for calibration to be successful Don’t set gain on no template controlsĬlick on “Start Run” Save file name in directory of your choice Default nomenclature has name of template file, date and version (1 for 1st run that day etc) With SYBR use “Melt” to look for primer-dimer or non-specific product Melt should start from acquiring temperature If you start the melt from annealing temp the melt may have a shoulder on it Use default settings, make sure you are acquiring data (blue dots) click on “Edit Profile” to change run parameters section to be altered is greyed out, click on “Hold Temperature” to change temperature, click on “Hold Time” to change time Always read manufacturer’s instructions regarding Hot start Taq activation timesĬlick on “Cycling” to adjust the cycling parameters Default cycle number is 40 To change a step click on the temperature and adjust then click on the time and adjustĭata is acquired each cycle (blue dots) No blue dots = no data = very bad Acquire at the end of the extension phase Click on “Aquiring to Cycling A” to select colours Move the colours you want from “Available Channels” to “Acquiring Channels” using arrows Use Green channel for SYBR green Insert user name, any notes (primer conc, primer sequence mix used etc) and volume required (10-25uL) This info is saved and can be printed in reports Click “Next” Select the rotor you are using, put the rotor into the machine with the locking ring attached The locking ring tick box must be ticked to proceed Click “Next” Getting Started…… Click on Rotor-Gene icon Click on advanced tab for step by step run set up Choose run template depending on chemistry Dual labeled probes = hydrolysis probes SYBR = intercalating dyes ![]() The world’s only real-time rotary thermo-optical analyser When self-collecting/ordering EXW from countries within or outside the European Union, 16% VAT will be retained as a deposit until we have received the corresponding confirmation of arrival/bill of delivery from the buyer.Corbett LIFE SCIENCE Getting Started on the Rotor-Gene-6000 Jennifer McMahon, PhD Note for international shipments: A proof of preference/EUR1 will not be issued by us. If transport costs are not specified, please ask separately for them.įor deliveries to countries outside the EU, an additional fee of 70,00€ for export is charged. Stated shipping costs are to be expected. Parcel services, forwarding agencies, self-pickup, delivery by Labexchange fleet. A partial delivery is possible on explicit request. The shipping time is calculated based on the position with the longest lead time. ![]() ![]() The effective dispatch times will be stated in the order confirmation.Īs a matter of principle, we are offering collective deliveries. The stated dispatch times are the shortest possible ones for each article. You are receiving only fully functional devices. The second-hand devices are verified by Labexchange Service GmbH before delivery. Status, terms of delivery and payment Verification of devices
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